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Neuroendocrinology Letters incl. Psychoneuroimmunology & Chronobiology


NeuroendocrinologyİLetters incl. Psychoneuroimmunology & Chronobiology,
ISSNİ0172ñ780X Copyrightİ©İ1998, 1999, 2000, 2001 NeuroendocrinologyİLetters

NEL VOL. 19 No. 2

1998; 19:79-85


Ultrastructural Demonstration of NADPH-Diaphorase, a Marker for Nitric Oxide Synthase in Hypothalamo-Neurohypophysial System, Hippocampus and Cerebral Cortex of the Rat After Ischemia
by Barbara Gajkowska and Michal Walski


An electron-microscopic histochemical study was performed in order to determine the expression pattern of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in hypothalamo-neurohypophysial system (HNS), hippocampus and cerebral cortex of rat's brain after ischemia. In basal conditions NADPH-d to be weakly expressed in small population of neuronal cells in HNS and hippocampus and in population of endothelial cells of capillary vessels in all examined regions of the brain. Short time after ischemia, upregulation NADPH-d expression was observed. NADPH-d was rapidly upregulated at 10 min and progressively increased 1-6 h after ischemia. A strong enzyme histochemical staining for NADPH-d was located in some neurosecretory neurons, astrocytes and cellular elements of capillary vessels of hypothalamus and in fibers, terminals, pituicytes and capillary vessels of neurohypophysis. The results suggest that increase in activity of NOS occurs during cerebral ischemia is involved in modulation of HNS. Also, the strong distribution of NADPH-d activity was found in capillary vessels, perivascular astrocytes and in some neurons associated with capillaries of hippocampus. The participation of NOS neurons and astrocytes in the changes in cerebral blood flow and microvascular function in ischemic tissue remain to be determined.

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