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Neuroendocrinology Letters Vol. 20 No. 5 Contents
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Neuroendocrinology Letters incl. Psychoneuroimmunology & Chronobiology, Research papers.
ISSN 0172–780X Copyright © 2000 Neuroendocrinology Letters

NEL Vol. 20 No. 5
Original Article
Full text pdf (152kb)
1999; 20:299–304
pii:NEL200599A02

Wavelength dependency of light-induced changes in rhythmic melatonin secretion from chicken pineal gland in vitro

by Valér Csernus, Péter Becher & Béla Mess

Keywords:
chicken pineal, in vitro, melatonin, light wavelength, circadian rhythm, perifusion

Abstract:
Avian pineals show rhythmic, circadian melatonin secretion pattern also in vitro. The phase of this rhythm can be modified by changing the illumination. Reversed in vitro illumination reverses the phase of the circadian melatonin rhythm in two days. In the present study the effect of the wavelength on the speed of the phase shift of the melatonin rhythm of the explanted chicken pineals was investigated in a dynamic in vitro bioassay. Chicken pineals were placed into perifusion columns and the tissue was exposed to reversed illumination through various light filters. Changes in the melatonin release during 4 day long experiments were studied. Clear differences in the speed of the reversal of the melatonin rhythm were observed as a function of the transmission spectrum of the light filters. The shorter the transmitted wavelength, the more rapid the phase shift was found. These data are in good agreement with earlier studies showing that the chicken pineal photo-pigment, pinopsin, is a blue light sensitive molecule. Our observation reveals that the blue light sensitive pigment is not only present but also fully functional in controlling the circadian biological clock in the chicken pineal gland.

 

Neuroendocrinology Letters incl Psychoneuroendocrinology, Chronobiology:
A peer-reviewed transdisciplinary Journal covering neuroendocrinology, psyhoneuroimmunology and chronobiology. Publishing basic and clinical research papers, review articles and state-of the art from the basic research and clinical research. E-mail: info@nel.edu
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