Neuroendocrinology Letters incl Psychoneuroimmunology & Chronobiology, Home
Neuroendocrinology Letters Vol. 20 No. 6 Contents
Contact us

Neuroendocrinology Letters incl. Psychoneuroimmunology & Chronobiology,
Season's Appreciations
ISSN 0172–780X Copyright © 2000 Neuroendocrinology Letters

NEL Vol. 20 No. 6
Original Article
1999; 20:365–372
Expression of NADPH-diaphorase (NOS) in rat hypothalamo-neurohypophysial system after ischemic and traumatic brain injury
by Barbara Gajkowska, Michal Walski & Hanna Olszewska-Badarczuk

hypothalamo–neurohypophysial system, rat, ultrastructure, NOS-histochemistry, brain injury

To investigate the sites of nitric oxide synthase (NOS) expression after brain injury, NADPH-d histochemistry was performed on the hypothalamo-neurohypophysial system (HNS) of adult rats several days after both ischemia and trauma. Electron-microscopic examination revealed the sites of formazan end-product of the diaphorase reaction in some neurons, astrocytes, cells and vascular pericytes, and in all activated microglial/macrophagal cells in perivascular and juxtaneuronal regions of hypothalamus. In neurohypophysis, positive NADPH-d staining was present in cytosol of many pituicytes, endothelial cells, pericytes and corresponding axonal endings. NADPH-d activity was also present in perivascular macrophages or microglial cells of neurohypophysis. Finally, we suppose that NOS expression and the consequent productions of nitric oxide could be involved in pathophysiology of HNS injury by ischemia and trauma, where activations inducible isoform of NOS especially, may contribute to a variety of neurogenerative processes. The imbalance in regulation of nitric oxide could disturb the physiological function of this neuroendocrine system.


Neuroendocrinology Letters incl Psychoneuroendocrinology, Chronobiology:
A peer-reviewed transdisciplinary Journal covering neuroendocrinology, psyhoneuroimmunology and chronobiology. Publishing basic and clinical research papers, review articles and state-of the art from the basic research and clinical research. E-mail:
Copyright © Neuroendocrinology Letters 2000 incl. Psychoneuroimmunology & Chronobiology
All rights reserved. No part may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or ortherwise, without prior written permission from the Editor-in-Chief: