Does leptin modulate immune and endocrine response
in the time of LPS-induced acute inflammation? by Wojciech Bik, Ewa Wolinska-Witort, Magdalena Chmielowska,
Elzbieta Rusiecka-Kuczalek & Boguslawa Baranowska
In many studies it has been reported, that leptin may play
an important role not only in the regulation of food intake
and body weight but can modify immune response. The aim of
our study was to estimate the effects of the administration
of exogenous leptin on serum concentration of proinflammatory
cytokines (interleukin 6-IL 6 and tumor necrosis factor alpha-TNF
a) and anti-inflammatory cytokine
(interleukin 10 IL 10) during LPS induced acute inflammation.
We also estimated leptins influence on pituitary, thyroid,
adrenal and gonadal hormones in response to lipopolisaccharyde
(LPS) induced acute inflammation.
METHODS: Male rats Wistar-Kyoto were divided into four groups,
which received respectively: placebo (0.9% NaCl), LPS, leptin
and leptin with LPS. The TNF a
and IL 6 serum concentrations were measured after 2 hours
and IL 10 after 4 hours. The pituitary, thyroid, adrenal and
gonadal hormones serum concentrations were measured after
2 and 4 hours. Cytokine concentrations were estimated using
ELISA tests and hormones concentrations using RIA tests.
RESULTS: Leptin did not have an effect on both cytokine responses
(proinflammatory and anti-inflammatory) in the time of LPS-induced
acute inflammation. Leptin enhanced LPS-induced increasing
of corticosterone secretion after 2 hours and decreased LPS-induced
inhibition of testosterone secretion after 4 hours.
CONCLUSIONS: Leptin can modulate hormone response during LPS-induced