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Neuroendocrinology Letters Vol. 22 No. 4 Contents
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Neuroendocrinology Letters incl. Psychoneuroimmunology & Chronobiology

NeuroendocrinologyŢLetters incl. Psychoneuroimmunology and Chronobiology, Editorial.
ISSNŢ0172˝780X CopyrightŢęŢ2001 NeuroendocrinologyŢLetters

NEL VOL. 22 No. 4
Original Article

Full text pdf (102kb)

2001; 22:238-242
pii: NEL220401A02
PMID: 11524629


The insulin, glucose and cholesterol level and activity of lysosomal enzymes in the course of the model alloxan diabetes
by Bozena Witek, Teodora Król, Adam Koataj, Ewa Ochwanowska, Iwona Stanisawska & Alina Slewa

Keywords:
Alloxan diabetes, insulin level, glycaemia level, cholesterol level, lysosomal enzymes, rabbits

Submitted: July 23, 2001
Accepted: July 30, 2001

Abstract:

OBJECTIVES: The study was carried out on fifty male rabbits of the New Zealand White breed. Diabetes was caused by a single, intravenous alloxan injection. Rabbits which had glycaemia 7th day after the alloxan administration higher than 11 millimol/litre were selected for the studies. They were divided into 5 groups: I ˝ control (without diabetes); II ˝ 3-week diabetes; III ˝ 6-week diabetes; IV ˝ 3-month diabetes; V ˝ 6-month diabetes.

METHODS: In control and experimental rabbits the activity of b-glucuronidase, N-acetyl-b-glucosaminidase, lysosomal acid phosphatase, leucine aminopeptidase, cathepsin D, and lysosomal arylesterase was determined in lysosomal fractions of the liver and kidney.

RESULTS: Alloxan caused lowering of the activity of all the investigated enzymes in the kidney and liver except lysosomal arylesterase.

CONCLUSION: Alloxan injection caused a significant increase in the activity of all the investigated enzymes. The advisable lysosomal enzymes may be useful for the monitoring of the course and effectiveness of diabetes therapy.

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