insulin, glucose and cholesterol level and activity of lysosomal
enzymes in the course of the model alloxan diabetes
by Bozena Witek, Teodora Król, Adam Koataj, Ewa
Ochwanowska, Iwona Stanisawska & Alina Slewa
Alloxan diabetes, insulin level, glycaemia
level, cholesterol level, lysosomal enzymes, rabbits
July 23, 2001
Accepted: July 30, 2001
OBJECTIVES: The study was carried out on fifty male rabbits
of the New Zealand White breed. Diabetes was caused by a single,
intravenous alloxan injection. Rabbits which had glycaemia
7th day after the alloxan administration higher than 11 millimol/litre
were selected for the studies. They were divided into 5 groups:
I ˝ control (without diabetes); II ˝ 3-week diabetes; III
˝ 6-week diabetes; IV ˝ 3-month diabetes; V ˝ 6-month diabetes.
In control and experimental rabbits the activity of b-glucuronidase,
acid phosphatase, leucine aminopeptidase, cathepsin D, and
lysosomal arylesterase was determined in lysosomal fractions
of the liver and kidney.
Alloxan caused lowering of the activity of all the investigated
enzymes in the kidney and liver except lysosomal arylesterase.
Alloxan injection caused a significant increase in the activity
of all the investigated enzymes. The advisable lysosomal enzymes
may be useful for the monitoring of the course and effectiveness
of diabetes therapy.