Neuroendocrinology Letters, Home
Vol. 22 No. 5 Contents
Contact us
Subscription info
Neuroendocrinology Letters incl. Psychoneuroimmunology & Chronobiology

NEUROENDOCRINOLOGY LETTERS
including Psychoneuroimmunology, Neuro
psychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology
ISSN 0172–780X

NEL VOL. 22 No. 5
Original Article


Full text pdf (81 kb)

2001; 22:337-341
pii: NEL220501A02


Relationship to the Proliferative Activity and Apoptosis of Mucous Epitheliocytes
by Igor Kvetnoy, Viktor Popuichiev, Ludmila Mikhina, Vladimir Anisimov, Vadim Yuzhakov, Sergey Konovalov, Nina Pogudina, Claudio Franceschi, Lucio Piantanelli, Giuliana Rossolini, Annamaria Zaia, Tatiana Kvetnaia,
Jose Hernandez-Yago & Jose Raphael Blesa

Keywords:
aging, diffuse neuroendocrine system, gut, proliferation, apoptosis

Submitted: July 15, 2001
Accepted: August 8, 2001

Abstract:

OBJECTIVES: Diffuse neuroendocrine system (DNES) cells regulate homeostasis via neurocrine, endocrine and paracrine mechanisms. Extensive effects of peptide hormones and biogenic amines necessitate studying of DNES cell biology in aging. In this connection, the functional morphology of gut neuroendocrine cells (NEC), proliferative activity and apoptosis of mucous epithelial cells in aging have been studied.

MATERIAL AND METHODS: The study was performed on BALB/c-nu mice of 4, 21 and 34 months of age. NEC, proliferative activity and apoptosis of mucous epitheliocytes in stomach and duodenum have been studied by histochemical, immunohistochemical and morphometrical methods.

RESULTS: The total number of NEC shows an increasing trend with advancing age. However, the different types of NEC elicit differential patterns. The total number of epithelial cell nuclei does not show any statistically significant difference during aging. The proliferative activity of mucous epitheliocytes also shows no difference among the three animal groups studied. On the contrary, the apoptotic index increases with advancing age.

CONCLUSIONS: The results demonstrate that various gut NEC show differential behavior with age and their time-courses are dependent on the site of location (stomach or duodenum). The picture seems quite complex to allow a comprehensive interpretation, nonetheless it gives us some useful indications for further investigation. In fact, since the gut does not show evident gross age-related physiological changes, modifications with age in specific biological parameters can suggest the key mechanisms of compensative regulatory processes possibly acting during aging.

 

__________________________________________________________
Copyright  Neuroendocrinology Letters 2001
All rights reserved. No part may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or ortherwise, without prior written permission from the Editor-in-Chief.
HWCounter.com