Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172780X
Vol.24 No.1/2, Feb-Apr 2003
receptor tissular localization
properties of hemerythrin of the sand worm Nereis diversicolor
Deloffre, Beatrice Salzet, Didier Vieau,
Jean-Claude Andries & Michel Salzet
de Neurobiologie des Rythmes, CNRS-UMR 7518, Université
Louis Pasteur, Strasbourg, FRANCE.
November 7, 2002 Accepted: November 14, 2002
hemerythrin, immunity, annelids, hematopoietic center,
To investigate the immune defense of the annelid Nereis diversicolor
and the key role of a oxygen-binding protein, the metalloprotein
MPII animals were subjected to bacteria infection.
AND RESULTS: Using RACE-PCR, we have cloned the complete cDNA
coding for the MPII related to the hemerythrin family in the
sand worm Hediste diversicolor. This cDNA (883 pb) codes for
a polypeptide of 119 amino acid residues with no signal peptide.
Previous works have identified this protein as a cadmium scavenger.
We here clearly demonstrated that this protein is also involved
in the worm defence towards bacteria growth by its iron scavenger
ability. This protein is expressed and produced in a haematopoietic
center that floats freely in the coelomic fluid before stored
in a particular hemocyte type: the granulocyte type 1. During
bacterial challenge, this protein contained in these cells
is discharged into the blood stream 3-4 hours after the infection
and remains active for approximately 10 hours. This time period
blocks progression of the pathogen and its attachment to tissues.
These results reflect that MPII in conjunction with others
partners like lysozyme act as defence molecule for the sand
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