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NEUROENDOCRINOLOGY LETTERS
including Psychoneuroimmunology, Neuropsychopharmacology,
Reproductive Medicine, Chronobiology
and Human Ethology, ISSN 0172–780X

NEL Vol.24 Nos.3/4, Jun-Aug 2003

ORIGINAL ARTICLE

Running Title:
Testosterone and gonadotropin levels in men with dementia

2003; 24:209214
pii: NEL243403A10
PMID: 14523359

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The effects of photoperiod on testes in rat:
A morphometric and immunohistochemical study

Ilter Kus 1, Nusret Akpolat 2, Hakan Oner 3, Ahmet Ayar 4,
Hýdýr Pekmez 1, Oguz Aslan Ozen 5 & Mustafa Sarsilmaz 1

1. Department of Anatomy, Faculty of Medicine, Firat University, Elazig;
2. Department of Pathology, Faculty of Medicine, Firat University, Elazig;
3. Department of Histology & Embryology, Faculty of Veterinary, Antalya University, Burdur;
4. Department of Pharmacology, Faculty of Medicine, Firat University, Elazig;
5. Department of Anatomy, Faculty of Medicine, Zonguldak Karaelmas University, Zonguldak;
TURKEY.

Submitted: September 19, 2002
Accepted: October 22, 2002

Key words:
photoperiod, testes, immunohistochemistry, rat

 

Abstract

OBJECTIVE: The aim of this study was to examine the effects of photoperiod on testes in rat.

DESIGN: For this purpose 21 male Wistar rats were used. Animals were divided into three groups. Control rats in group I were kept under 12 hrs light: 12 hrs dark conditions (12L: 12D) for 10 weeks. Animals in group II were exposed to long photoperiods (18L: 6D), while rats in group III were exposed to short photoperiods (6L: 18D) for 10 weeks. At the end of the experiment, all animals were killed by decapitation and blood samples were obtained. Serum testosterone levels were determined with the use of a chemiluminescent enzyme immunoassay. The testes of all rats were removed and weighed. Testicular tissue was processed semiquantitative evaluation of immunohistochemical testosterone staining. Intensity of immunostaining was determined on a scale between 0 (no staining) and 5 (heavy staining). For morphometric comparison, diameters of seminiferous tubules in each group were measured.

RESULTS: In rats exposed to long photoperiods, testicular weights, diameters of seminiferous tubules and serum testosterone levels were significantly increased as compared to those in control rats. Whereas, exposure of rats to short photoperiods resulted in significantly decrease of testicular weights, diameters of seminiferous tubules and serum testosterone levels as compared to those in control rats and rats maintained in long photoperiods. Immunostaining of testosterone was moderate (3+) in control rats, heavy (5+) in rats exposed to long photoperiods and minimal (1+) in rats exposed to short photoperiods.

CONCLUSIONS: The results of our study indicate that testicular functions increase after exposure to long photoperiods and decrease after exposure to short photoperiods.

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Society of Integrated Sciences
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