OBJECTIVES: Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated.
METHODS: The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes.
RESULTS: Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney.
CONCLUSION: Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups.
ISSN: 0172-780X |
ISSN-L: 0172-780X |
eISSN 2354-4716
www.nel.edu Editor-in-Chief: Peter G. Fedor-Freybergh
www.nel.edu Editor-in-Chief: Peter G. Fedor-Freybergh