Search NEL Online

Journal Article 2014; 35(6): 454-462 PubMed PMID: 25433849 Citation  Keywords:  Acetylglucosaminidase:metabolism, Alzheimer Disease:drug therapy, Animals, Antioxidants:pharmacology, CA1 Region, Hippocampal:drug effects, Cognition Disorders:drug therapy, Disease Models, Animal, Indoles:pharmacology, Male, Malondialdehyde:metabolism, M.   

BACKGROUND: The idea of antioxidant therapy attenuating Alzheimer disease (AD) neuropathology starts to be attractive. Animal models are often used in these studies. An AD-like model of trimethyltin (TMT)-induced neurodegeneration, targeting the hippocampus, involves neuronal cell death and cognitive impairment.

OBJECTIVES: Effect of the pyridoindole SMe1EC2 (3×50 mg/kg) and vitamin C (3×50mg/kg) was analyzed in the model of TMT-induced (8 mg/kg) neurodegeneration.

METHODS: The study was focused on the effect of the antioxidants tested on learning performance in the Morris water maze (MWM) on days 21-25 after TMT administration, on biochemical variables - malondyaldehyde (MDA) and lysosomal enzyme NAGA in brain cortex and blood serum, and on pyramidal cell number in the CA1 area of the hippocampus on day 31 after TMT administration in adult male Wistar rats (n=32).

RESULTS: Critical deterioration of learning performance was observed due to the TMT administration in the MWM. Further, apparent reduction of pyramidal cell number to 21% in the CA1 area of the hippocampus, increased MDA and NAGA activity in serum and increased NAGA activity in the cortex were determined contrary to controls. In serum, an increase of MDA level was prevented by both antioxidants tested without any effect on NAGA activity. SMe1EC2 apparently preserved pyramidal cell viability in the CA1 area. Both substances tested failed to ameliorate the detrimental effect of TMT on spatial memory.

CONCLUSION: The biochemical and morphometrical findings suggest that reduction of oxidative stress may play a role in AD-like neurodegeneration. Different doses and timing of SMe1EC2 administration might bring improvement in next learning performance....

Read abstract  Full text PDF

Journal Article 2002; 23(2): 105-108 PubMed PMID: 12011794 Citation  Keywords:  Acetylglucosaminidase:metabolism, Animals, Anti-Inflammatory Agents:pharmacology, Antigens, CD13:metabolism, Cathepsin D:metabolism, Cathepsin L, Cathepsins:metabolism, Cysteine Endopeptidases, Enzyme Activation:drug effects, Homeostasis:drug effects, Hyd.   

OBJECTIVES: Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w. of hydrocortisone injection for 4 and 8 days.

METHODS: The homogenates of the liver and kidney were subjected to differentiated centrifuging and determination of studied enzymes.

RESULTS: Injection of hydrocortisone caused an increase in the activity of all investigated lysosomal enzymes in the liver and kidney of mice.

CONCLUSION: The reactions of selected mice were stronger in comparison with unselected ones. The highest increase in the activity investigated enzymes was observed after 8 days of hydrocortisone injection....

Read abstract

Journal Article 2001; 22(3): 181-185 PubMed PMID: 11449196 Citation  Keywords:  Acetylglucosaminidase:metabolism, Animals, Antigens, CD13:metabolism, Cathepsin D:metabolism, Cathepsin L, Cathepsins:metabolism, Cysteine Endopeptidases, Female, Glucuronidase:metabolism, Kidney:enzymology, Kinetics, Lipase:metabolism, Liver:enzymology, .   

OBJECTIVES: Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated.

METHODS: The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes.

RESULTS: Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney.

CONCLUSION: Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups....

Read abstract  Full text PDF