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Effect of hydrocortisone on the activity of some lysosomal enzymes in mice.

Witek B, Ochwanowska E, Slewa A, Kolataj A.
Journal Article 2002; 23(2): 105-108 PubMed PMID: 12011794 Citation  Keywords:  Acetylglucosaminidase:metabolism, Animals, Anti-Inflammatory Agents:pharmacology, Antigens, CD13:metabolism, Cathepsin D:metabolism, Cathepsin L, Cathepsins:metabolism, Cysteine Endopeptidases, Enzyme Activation:drug effects, Homeostasis:drug effects, Hyd.   

OBJECTIVES: Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w. of hydrocortisone injection for 4 and 8 days.

METHODS: The homogenates of the liver and kidney were subjected to differentiated centrifuging and determination of studied enzymes.

RESULTS: Injection of hydrocortisone caused an increase in the activity of all investigated lysosomal enzymes in the liver and kidney of mice.

CONCLUSION: The reactions of selected mice were stronger in comparison with unselected ones. The highest increase in the activity investigated enzymes was observed after 8 days of hydrocortisone injection....

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Effect of melatonin administration on activities of some lysosomal enzymes in the mouse.

Witek B, Ochwanowska E, Kolataj A, Slewa A, Stanislawska I.
Journal Article 2001; 22(3): 181-185 PubMed PMID: 11449196 Citation  Keywords:  Acetylglucosaminidase:metabolism, Animals, Antigens, CD13:metabolism, Cathepsin D:metabolism, Cathepsin L, Cathepsins:metabolism, Cysteine Endopeptidases, Female, Glucuronidase:metabolism, Kidney:enzymology, Kinetics, Lipase:metabolism, Liver:enzymology, .   

OBJECTIVES: Changes in the activity of beta-glucuronidase, N-acetyl-beta-glucosaminidase, cathepsin D and L, alanine aminopeptidase and lysosomal acid lipase in lysosomal fractions of the liver and kidneys of mice, which were administered 20 mg/kg b.w. of exogenous melatonin (N-acetyl-5-methoxytryptamine) for 7 and 14 days were investigated.

METHODS: The slices of the liver and kidney were homogenized in 0.1M phosphate buffer, pH 7.0. Homogenates were subjected to differentiated centrifuging and determination of studied enzymes.

RESULTS: Melatonin caused lowering of the activity of all the investigated lysosomal enzymes in the liver and kidney.

CONCLUSION: Administration of melatonin was caused the lowering of the activity of the investigated lysosomal enzymes in comparison with values in control groups....

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