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SUMO negatively regulates BACE expression.

Fang H, Du X, Meng F, Zhou J.
Journal Article 2011; 32(3): 313-316 PubMed PMID: 21712774 Citation  Keywords:  Amyloid Precursor Protein Secretases:biosynthesis, Amyloid beta-Peptides:biosynthesis, Amyloid beta-Protein Precursor:biosynthesis, Aspartic Acid Endopeptidases:biosynthesis, Cell Line, Cells, Cultured, DNA:biosynthesis, Humans, Luciferases:genetics, RNA,.   

OBJECTIVE: The effect of SUMO on the promoter activity and mRNA expression of BACE gene was investigated to find the connection between sumoylation and APP processing.

METHODS: The BACE promoter activity was measured by reporter gene analysis and BACE mRNA level was investigated using real-time RT-PCR method.

RESULTS: BACE gene promoter activity was inhibited by the over-expression of SUMO proteins and was blocked by disrupting the SP1 site. Endogenous BACE mRNA level was also negatively regulated by the induction of SUMO proteins. Using a specific inhitor of SP1, BACE promoter activity was coordinately inhibited.

CONCLUSIONS: SUMO negatively regulates the BACE expression and SP1 is involved in the process....

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