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Comparative Study 2007; 28(Suppl 2): 17-18 PubMed PMID: 17558368 Citation  Keywords:  Aminoglycosides:administration & dosage, Anti-Bacterial Agents:administration & dosage, Carbapenems:administration & dosage, Child, Child, Preschool, Cross Infection:drug therapy, Drug Therapy, Combination, Glycopeptides:administration & dosage, Humans, I.   

: We have been unable to document a benefit of a combination of aminoglycosides with betalactam or carbapenem antibiotics in nosocomial meningitis. This was similar to the cases of sepsis, where survival of patients did not improve with combination therapy. Combination therapy did not increase the chance of appropriateness of the therapy. 30% of those on combination therapy were considered as inappropriately treated in comparison to 2.8% of those on monotherapy (p<0.01)....

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Journal Article 2007; 28(Suppl 2): 15-16 PubMed PMID: 17558367 Citation  Keywords:  Acinetobacter Infections:drug therapy, Acinetobacter baumannii:immunology, Anti-Bacterial Agents:therapeutic use, Central Nervous System:abnormalities, Child, Child, Preschool, Cross Infection:drug therapy, Humans, Infant, Newborn, Infant, Very Low Birth .   

: Twenty five (25) cases of nosocomial postsurgical meningitis due to Acinetobacter baumannii meningitis were compared to other 146 cases of meningitis after surgery caused by other pathogens. Prior neurosurgical ventriculo-peritoneal shunt insertion and CNS abnormality as well as very low birth weight were significant risk factors for acquisition of Acinetobacter baumannii meningitis. Mortality - 40% among children with nosocomial meningitis was unacceptably high and significantly higher than among meningitis caused by microorganisms other than Acinetobacter baumannii....

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Comparative Study 2007; 28(Suppl 2): 11-14 PubMed PMID: 17558366 Citation  Keywords:  Child, Child, Preschool, Cross Infection:epidemiology, Data Collection, Gram-Negative Bacterial Infections:epidemiology, Humans, Infant, Infant, Newborn, Meningitis, Bacterial:epidemiology, Risk Factors, Slovakia:epidemiology, Staphylococcal Infections:ep.   

: In 1993-1998 and in 1999-2004 we have performed two surveys of pediatric bacterial meningitis in all 8 neurosurgery/pediatrics and infectious diseases departments in Slovakia. We have detected 101 and another 54 cases with attributable mortality of 15% and sequellae in 18%....

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Journal Article 2006; 27(Suppl 1): 41-45 PubMed PMID: 16804516 Citation  Keywords:  Adult, Antibodies, Bacterial:analysis, Case-Control Studies, Female, Helicobacter Infections:complications, Helicobacter pylori:immunology, Humans, Immunoglobulin A:analysis, Male, Middle Aged, Thyroiditis, Autoimmune:immunology,.   

OBJECTIVES: In this study we examined the anti-Helicobacter pylori (anti-H. pylori) antibodies in patients with autoimmune thyroiditis, with and without different polyglandular involvement, and in healthy controls.

MATERIAL & METHODS: Patients with autoimmune thyroiditis (AT) were divided into three groups: Group A: 23 patients with isolated AT, Group B: 30 patients with AT as a part of polyglandular activation of autoimmunity, and Group C: 7 patients with AT as a part of autoimmune polyglandular syndrome type II. Thirty healthy individuals served as controls (Group D). Anti-H. pylori antibodies were determined first by ELISA for classes IgG, IgA, and IgM, and subsequently by immunoblot for classes IgG and IgA.

RESULTS: ELISA: The number of patients with IgA antibodies in Group A (39%) and Group B (30%) differed significantly from controls (7%, p<0.05). Immunoblot: Anti-CagA antibodies were found in 13% of patients in Group A, 7% of Group B, 0% of Group C, and 20% of Group D. A higher seroprevalence, as compared to controls, was found for IgG to the VacA (p=0.01), 30 kDa (p=0.001), and 17 kDa (p=0.008) antigens in Group A and for IgG to the 30 kDa antigen in Group C (p=0.037). A significantly higher seroprevalence, as compared to controls, was likewise found for IgA to the 17 kDa antigen in Group A (p=0.015).

CONCLUSIONS: A different distribution of antibodies to H. pylori antigens was found in patients with isolated AT compared to patients with AT coupled with a polyglandular syndrome....

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Clinical Trial 2004; 25(5): 373-380 PubMed PMID: 15580173 Citation  Keywords:  Adolescent, Adult, Anti-Bacterial Agents:therapeutic use, Combined Modality Therapy, Female, Gestational Age, Humans, Obstetric Labor, Premature:therapy, Pregnancy, Pregnancy Outcome, Tocolysis, Treatment Outcome,.   

OBJECTIVE: The aim of this study was to determine: a) to what extent application of antibiotics enhances efficiency of tocolysis; b) whether the duration of antibiotic treatment influences its efficacy; c) what criterion is decisive for efficacy of tocolysis assisted by antibiotic therapy.

MATERIALS AND METHODS: 223 successive women with unifetal pregnancies, aged 17 to 42 (average age 27.3), admitted to the Department of Gynecology and Obstetrics of the JU Medical College in the period from January 1, 1999 to September 9, 2001, were enrolled in the study. Using clinical methods such as: assessment of contractile activity of the uterus, of uterine cervix and membranes, presence of bleeding and other clinical symptoms, these women were diagnosed with imminent preterm labour.

CONCLUSIONS: Antibiotic treatment enhances the efficacy of tocolysis, influencing the time of prolonging pregnancy in imminent preterm delivery. This synergistic effect is clinically crucial as in this way significantly larger number of children will be born at term and also a number of premature neonates who survive may be increased....

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Journal Article 2003; 24(5): 350-354 PubMed PMID: 14647012 Citation  Keywords:  Animals, Anti-Bacterial Agents:pharmacology, Antioxidants:pharmacology, Female, Graft Survival, Malondialdehyde:blood, Melatonin:pharmacology, Necrosis, Ovary:metabolism, Oxytetracycline:pharmacology, Peritoneal Cavity, Rats, Rats, Wistar,.   

OBJECTIVES: To comparatively investigate the effects melatonin and oxytetracycline as antioxidants on autologous intraperitoneal ovary transplantation in rats.

SETTING AND DESIGN: 28 adult female Wistar rats with regular estrous cycles were randomly divided into four groups. Group 1 (n=7) was the control group; Group 2 (n=7) bilateral oophorectomy and intraperitoneal transplantation group; and Groups 3 (n=7) and 4 (n=7) firstly received 20 mg/kg/IP melatonin and 10 mg/kg/IP oxytetracycline., respectively and fifteen minutes after that, they had bilateral oophorectomy and intraperitoneal transplantation. Ovarian tissue and blood malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) levels were determined. Vaginal cytology and ovarian necrosis were histopathologically investigated. Kruskall Wallis analysis of variance was used in the statistical analysis of data.

RESULTS: Ovarian necrosis, tissue MDA and plasma MDA levels were significantly higher in Group 2 compared to the Groups 1, 3 and 4 (p<0.03). SOD and GSHPx levels were significantly lower in group 2 compared to the groups 1, 3 and 4 (p<0.03). Melatonin was found to be more effective on ovarian necrosis and tissue MDA level than the use of oxytetracycline following autologous intraperitoneal ovary transplantation (p<0.03). There was a significant positive correlation between tissue MDA level and ovarian necrosis and a very strong negative correlation between tissue MDA and SOD level (rs = +/-0.9, n=28, p=0.000).

CONCLUSION: Although both melatonin and oxytetracycline were found to be effective in autologous intraperitoneal pelvic ovary transplantation, melatonin is more effective against both the ovarian necrosis and tissue MDA level in rats....

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Journal Article 2003; 24(1-2): 39-45 PubMed PMID: 12743530 Citation  Keywords:  Amino Acid Sequence, Animals, Annelida:chemistry, Anti-Bacterial Agents, Bacteria:drug effects, Cloning, Molecular, DNA, Complementary:biosynthesis, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Escherichia coli:drug effects, Gra.   

OBJECTIVES: To investigate the immune defense of the annelid Nereis diversicolor and the key role of a oxygen-binding protein, the metalloprotein MPII animals were subjected to bacteria infection.

METHODS AND RESULTS: Using RACE-PCR, we have cloned the complete cDNA coding for the MPII related to the hemerythrin family in the sand worm Hediste diversicolor. This cDNA (883 pb) codes for a polypeptide of 119 amino acid residues with no signal peptide. Previous works have identified this protein as a cadmium scavenger. We here clearly demonstrated that this protein is also involved in the worm defence towards bacteria growth by its iron scavenger ability. This protein is expressed and produced in a haematopoietic center that floats freely in the coelomic fluid before stored in a particular hemocyte type: the granulocyte type 1. During bacterial challenge, this protein contained in these cells is discharged into the blood stream 3-4 hours after the infection and remains active for approximately 10 hours. This time period blocks progression of the pathogen and its attachment to tissues.

CONCLUSION: These results reflect that MPII in conjunction with others partners like lysozyme act as defence molecule for the sand worm....

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