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Journal Article 2006; 27(Suppl 2): 61-64 PubMed PMID: 17159781 Citation  Keywords:  Acquired Immunodeficiency Syndrome:microbiology, Animals, Bacteria:isolation & purification, Colon:microbiology, Colony Count, Microbial, Endocytosis, Female, HIV-1, HL-60 Cells, Humans, Male, Microbial Viability, Models, Biological, Rabbits,.   

OBJECTIVES: Bacteria purified from the intestinal tract of HIV/AIDS patients were tested for the capacity to be internalised by cells of the HL-60 cell line. Secondly, the bacteria have been applied to the rabbit's colon in order to test their pathogenic ability.

RESULTS: The ability of the bacteria to be internalised by HL-60 cells was found to be very expressive. For a more complex biological characterisation of internalised bacteria, these were applied in 6-day intervals per rectum to NZB x CA rabbits during 8 months. The administered bacteria were detected by dot blot hybridisation using HIV-1 PCR probes in the rabbits' intestinal tract after 2 months. No histological and pathological changes were recorded in the gastrointestinal epithelial cells of rabbits.

CONCLUSIONS: The applied bacteria of HIV/AIDS patients were not colonised in the rabbit's colon and disappeared after 2 months. Differences between HL-60 and rabbit model are discussed....

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