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Journal Article 2007; 28(5): 591-595 PubMed PMID: 17984947 Citation  Keywords:  Acquired Immunodeficiency Syndrome:drug therapy, Anti-Retroviral Agents:therapeutic use, Base Sequence, Citrobacter freundii:genetics, DNA, Bacterial:analysis, Enterobacter aerogenes:genetics, Enterobacteriaceae:genetics, Genes, Bacterial:genetics, Genes,.   

OBJECTIVES: Bacterial DNA isolated from the intestinal tract of 11 American and 30 Slovak HIV/AIDS patients were analyzed by colony and dot blot hybridization assay for HIV-1 specific sequences. Secondly, PCR using primers specific for the HIV-1 gag, pol and env genes for detection of HIV-1 sequences in these DNA were performed.

RESULTS: Intestinal bacteria DNA of HIV/AIDS patients hybridized in colony and dot blot hybridization assay for HIV-1 specific sequences. PCR products synthesized on specific primers of HIV-1 gag (115 bp), env (142 bp), pol-env (1484) genes were found to be for more than 90% homologous to the corresponding sequence in HIV-1.

CONCLUSIONS: Intestinal bacteria of HIV/AIDS patients are bearing sequences for more than 90% identical with those of HIV-1....

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