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Journal Article 2005; 26(4): 342-346 PubMed PMID: 16136012 Citation  Keywords:  Abortion, Spontaneous:blood, Adrenocorticotropic Hormone:blood, Cystinyl Aminopeptidase:blood, Female, Homeostasis:physiology, Humans, Hypothalamo-Hypophyseal System:physiology, Pituitary-Adrenal System:physiology, Pregnancy, Pregnancy Trimester, First,.   

INTRODUCTION: The labor at term finishes normal pregnancy. Both labor at term and first trimester spontaneous abortion are connected with increasing cytotoxic immune response within decidua. Th1 cytokines including IL-2 and INF-gamma are able to exert an effect on HPA axis and result in ACTH secretion. Oxytocinase serum level during pregnancy rises with the fetal development and arrest of oxytocinase serum growth might indicate the its development impairment, what might result in spontaneous abortion.

MATERIAL AND METHODS: The study group consisted of 27 patients with clinical symptoms of missed abortion. A control group consisted of 89 pregnant women, who were successfully treated because of infertility. Immunoassay was used to measure ACTH plasma concentration. Oxytocinase plasma activity was established using l-cystine-di-beta-naphthylamide as a substrate.

RESULTS: In the present study, significant increase in ACTH plasma concentration was observed during first trimester of spontaneous abortion. These patients were not characterized by significant increase of oxytocinase plasma level.

CONCLUSIONS: The observed ACTH rise during spontaneous abortion might be also related to the alterations at the maternal-fetal interface and the response of HPA axis to the growing cytotoxic activity....

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Journal Article 2005; 26(4): 347-350 PubMed PMID: 16136011 Citation  Keywords:  Adrenocorticotropic Hormone:blood, Adult, Follicle Stimulating Hormone:blood, Humans, Hydrocortisone:blood, Infertility, Male:blood, Leydig Cells:metabolism, Luteinizing Hormone:blood, Male, Sperm Count, Stress, Psychological:blood, Testosterone:biosynthe.   

INTRODUCTION: Emotional stress connected with work or, for example, depressive reaction to infertility, is one of frequent causes of decreased quality of semen. An objective examination of this factor is difficult because stress depends also on patient's emotionality. One of the ways to study this problem is evaluating the relation between ACTH level and selected semen parameters.

MATERIAL AND METHODS: The study was based on results of the examination of 83 men from couples treated for infertility in the Gynecology and Infertility Clinic of the Jagiellonian University in Krakow. Determination of ACTH, FSH, LH, cortisol, testosterone and androstendion was performed using ELISA.

RESULTS: Lower average values of semen volume, sperm cells concentration and A sperm cell mobility and higher value of D sperm cells mobility were identified in the group of men from couples treated for infertility with ACTH level >30 pg/ml in comparison to the WHO standards and the control group with ACTH <30 pg/ml. Higher ACTH and cortisol levels were detected in the group of men with the sperm count <10 mln/mL in comparison to the control group with the sperm count >20 mln/mL. Low and statistically insignificant correlation was observed between androstendion and testosterone levels, what might suggest the reduction of testosterone synthesis in this group.

CONCLUSIONS: Our results might indicate that the rise of ACTH and cortisol inhibit the conversion of adrostendion into testosterone in Leydig-cells resulting in the rise of androstendion, drop of testosterone production and lower average values of semen volume, sperm cells concentration and A sperm cell mobility and higher value of D sperm cells mobility....

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Journal Article 2005; 26(4): 351-354 PubMed PMID: 16136010 Citation  Keywords:  Acute Disease, Adult, Anxiety:blood, Humans, Hydrocortisone:blood, Male, Personality:physiology, Social Behavior, Stress, Psychological:blood,.   

OBJECTIVES: Traditionally, it has been hypothesized that highly anxious/emotionally reactive subjects may have exaggerated social stress response. We examined the relationship between self-reported anxiety, emotional reactivity, and social stress response.

METHODS: We investigated the relationship between personality scales of trait-state anxiety, subjective autonomic reactivity, and salivary cortisol levels before and after social stress exposure (Trier Social Stress Test) in 20 men.

RESULTS: Significant positive correlations between anxiety, subjective autonomic reactivity, and basal cortisol levels were observed, while neither anxiety nor subjective autonomic reactivity was correlated with social stress-induced cortisol elevation.

CONCLUSIONS: The present results indicate (i) subjects with higher degrees of trait anxiety/subjective autonomic reactivity have higher basal cortisol levels, and (ii) in contrast to the traditional view, anxious personality is not strongly associated with exaggerated cortisol response to social stress....

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Journal Article 2005; 26(4): 355-360 PubMed PMID: 16136009 Citation  Keywords:  Adaptation, Psychological, Adult, Aggression:physiology, Female, Hostility, Humans, Male, Middle Aged, Personality:physiology, Reference Values, Stress, Psychological:blood, Testosterone:blood,.   

OBJECTIVES: Many studies assessing the role of sex hormones, like testosterone, on stress and hostility factors have been primarily conducted in selected atypical populations such as violent criminals as well as androgen users and abusers. Therefore, the main aim of the current study was to investigate the association between testosterone levels and two psychosocial variables: stress and hostility in a cohort of healthy individuals who were members of a health maintenance organization (HMO).

METHODS: At five quarterly visits, psychosocial scales and blood draws were collected. Psychological stress was measured by using several scales that assessed different types of stress, including daily hassles, major life events and perceived stress. Similarly, different aspects of hostility were measured, among them cynicism, hostile affect and aggressive responding. Plasma collected from each visit was used for testosterone level determinations.

RESULTS: Testosterone levels were significantly associated with stress in both males and females. However, whereas this association exhibited a "threshold effect" in males, it demonstrated a direct and continuous linear relationship between these variables in females. Hostility was not correlated with testosterone levels in neither males nor females.

CONCLUSIONS: These results suggest that testosterone levels in normal males and females may be more reflective of an intricate balance between physiological responding and emotional coping to stressors than the hostility profile of the individual....

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Journal Article 2005; 26(4): 361-366 PubMed PMID: 16136008 Citation  Keywords:  Adrenocorticotropic Hormone:blood, Adult, Exercise:physiology, Female, Humans, Hydrocortisone:blood, Immune System:physiology, Immunoglobulin A:blood, Immunoglobulin G:blood, Immunoglobulin M:blood, Sports, Stress, Physiological:blood,.   

BACKGROUND: Despite the numerous studies controversial results exist in specific immune response to exercise. The aim of this study was to determining the differences in the humoral immune parameters, serum ACTH and cortisol levels existing between sportswomen and sedentary subjects and the effect of acute aerobic and anaerobic exercise on these parameters.

MATERIALS/METHODS: 40 sportswomen (Groups 1 and 2) and 20 sedentary women (Group 3) were enrolled and Group 1 performed aerobic exercise on a treadmill for 30 minutes while the Group 2 was subjected to the Wingate effort test for 30 seconds. Before exercising (at 8.30 a.m), immediately after the exercise, and 4 hours, days 2 and 5 days after exercising blood samples were obtained and the levels of IgA, IgG, IgM, complement 3 (C3) and complement 4 (C4) were determined turbidometrically.

RESULTS: Before exercise: the means of IgA and IgG values in the G1 and G2 groups were higher than the Group G3, and the mean cortisol levels in the sedentary group was significantly higher (p<0.05). After Exercise: Whereas the C3 and C4 levels decreased significantly at the end of the exercise in Group 1 and 2 (p<0.05), the IgA, IgG (p<0.05) and IgM levels (p<0.01) at the 4th and 5th determinations were observed to be significantly higher in only the Group 1. The cortisol and ACTH levels were found to have increased significantly (p<0.05) in the Group 1. In Group 2, no changes were observed in the IgA, IgG and IgM levels.

CONCLUSIONS: We conclude that regular and moderate exercise has favorable effects on the immune system by increasing immunoglobulins which are potent protective factors....

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Journal Article 2005; 26(4): 367-372 PubMed PMID: 16136007 Citation  Keywords:  Animals, Hypothalamo-Hypophyseal System:metabolism, In Vitro Techniques, Male, Neurokinin A:analogs & derivatives, Neurokinin-1 Receptor Antagonists, Neurotransmitter Agents:pharmacology, Peptide Fragments:pharmacology, Rats, Rats, Wistar, Receptors, Neur.   

OBJECTIVES: Present experiments were undertaken to study the influence of peptide NK-1 and NK-2 receptor agonists and antagonists as well as substance P and neurokinin A (the natural ligands for these tachykinin receptors) on vasopressin (AVP) secretion from the rat hypothalamo-neurohypophysial (HN) system in vitro.

RESULTS: The results showed that both substance P and highly selective tachykinin NK-1 receptor agonist, i.e., [Sar9,Met(O2)11]-Substance P, enhanced significantly AVP secretion, while the NK-1 receptor antagonist (Tyr6,D-Phe7,D-His9)-Substance P (6-11)--sendide--was found to antagonize the substance P-induced hormone release from isolated rat HN system (all peptides at the concentration of 10(-7) M/L). The NK-2 receptor selective agonist (beta-Ala8)-Neurokinin A (4-10) was essentially inactive in modifying AVP release from the rat HN system in vitro, while neurokinin A (the natural ligand for this tachykinin receptor) was found to stimulate the AVP release; this effect of neurokinin A has been diminished by the NK-2 receptor antagonist (Tyr5,D-Trp(6,8,9),Lys-NH2(10))-Neurokinin A (4-10).

CONCLUSION: The present data indicate a role for tachykinin NK-1 (and possibly for NK-2) receptors in tachykinin-mediated stimulation of AVP secretion from the rat HN system in vitro....

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Journal Article 2005; 26(4): 373-376 PubMed PMID: 16136006 Citation  Keywords:  Adenoma:blood supply, Apoptosis, Humans, Hyperplasia, Immunoenzyme Techniques, Neovascularization, Pathologic:metabolism, Nitric Oxide Synthase Type II:metabolism, Nitric Oxide Synthase Type III:metabolism, Parathyroid Glands:blood supply, Parathyroid Neo.   

OBJECTIVE: The aim of the presented study was to perform the immunohistochemical detection of endothelial (eNOS) and inducible (iNOS) isoform of nitric oxide synthase in the adenomatous and hyperplastic parathyroid gland in relation to the apoptotic process.

DESIGN AND SETTING: Tissue samples from 12 patients with parathyroid gland adenoma (PGA) and 10 patients with secondary parathyroid gland hyperplasia (PGH) were collected during surgery at the Department of Otorhinolaryngology and Head and Neck Surgery of The First Faculty of Medicine in Prague.

METHODS: Three-step immunoperoxidase reaction on acetone-fixed cryostat sections was performed using both polyclonal and monoclonal antibodies against eNOS and iNOS. The detection of apoptotic cells was done using antibody against cleaved caspase-3 as an apoptotic marker.

RESULTS: The immunoreactivity to eNOS antibody was observed in the endothelial lining of vessels in PGA, PGH and in the rim of normal parathyroid gland adjacent to PGA sample. Variable expression of eNOS was confirmed in arteries, arterioles, capillaries and veins in the glandular parenchyma as well as in the surrounding connective tissue. There was no iNOS immunoreactive cell detected in any examined sample. No apoptotic cells were detected.

MAIN RESULT: Our findings confirm that eNOS is regularly expressed in the vasculature of PGA and PGH.

CONCLUSION: eNOS observed in the vasculature of the enlarged parathyroid glands can serve as a factor that contributes to the viability of hypertrophic pathologic tissue. The lack of stimulating signals may be a reason for negative iNOS detection and negligible apoptotic rate....

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Journal Article 2005; 26(4): 377-382 PubMed PMID: 16136005 Citation  Keywords:  Animals, Copper:metabolism, Cyclic AMP:metabolism, Female, Gonadotropin-Releasing Hormone:metabolism, Hydrolysis, Inositol Phosphates:metabolism, Luteinizing Hormone:secretion, Nickel:metabolism, Phosphatidylinositols:metabolism, Pituitary Gland, Anterior.   

: Gonadotropin releasing hormone (GnRH) is an essential factor in the regulation of synthesis and release of pituitary gonadotropins. After binding to specific receptors and coupling with G proteins, it triggers the intracellular signaling involving the synthesis of inositol phosphates and diacylglycerol. Previously we have showed that certain metal complexes with GnRH, i.e. copper (Cu-GnRH) and nickel (Ni-GnRH) are able to bind to the GnRH receptors. The intracellular signalling of these complexes, however, has not been yet elucidated. In this experiment, the ability of the Cu-GnRH and Ni-GnRH complexes to modulate cAMP synthesis and phosphoinositols formation in the pig anterior pituitary cells in vitro was studied. The native GnRH and its metal complexes stimulated the luteinizing hormone (LH) release, but only the effect of Cu-GnRH was found to be a dose-dependent. The metal complexes did not significantly influence inositol phosphates accumulation, while their effect on cAMP synthesis was significantly more potent than that of GnRH alone. We conclude that the Cu-GnRH and Ni-GnRH complexes increase LH release in the porcine pituitary cells although their intracellular signaling is different from that of the native GnRH. It seems that metal complexes with GnRH deserve more attention in further studies....

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