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Journal Article 2012; 33(2): 177-182 PubMed PMID: 22592198 Citation  Keywords:  Action Potentials:drug effects, Animals, Bone Marrow Cells:drug effects, Cell Differentiation:drug effects, Cells, Cultured, Male, Mice, Mice, Inbred C57BL, Nerve Growth Factor:pharmacology, Neurofilament Proteins:metabolism, Neurons:metabolism, Stromal C.   

OBJECTIVE: Nerve growth factor (NGF) triggers long-term neuronal excitability. We examined its effect on murine bone marrow stromal cells (BMSC)-derived neurons.

METHODS: With an optimal differentiation protocol, BMSCs were differentiated into neurons in culture. To confirm the probability of differentiation of BMSC into neuron, the expression of neuronal marker protein, neurofilament, was examined by immunocytochemistry. To examine the electrophysiological properties of BMSC-derived neurons, the field potentials were recorded either from nontreated (control) BMSC-derived neurons or from BMSC-derived neurons after the treatment with NGF by using extracellular recording techniques.

RESULTS: Most BMSC-derived neurons showed spontaneous discharges whose amplitudes were up to 2 mV. When NGF at a concentration of 100 ng/ml was applied to BMSC-derived neurons, the amplitudes of discrete field potentials were gradually enlarged within 1 min after NGF application and peaked 3 min later (20-fold the size of control). However, the enlargement of the amplitudes of field potentials almost disappeared 5 min after NGF application.

CONCLUSION: This finding indicates that neuronal cells derived from murine BMSCs generate discrete field potential activities spontaneously and that NGF has the effect of enlarging transient, but not sustained, electrical activity of BMSC-derived neurons....

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Journal Article 2011; 32(1): 25-33 PubMed PMID: 21407154 Citation  Keywords:  Animals, Astrocytes:immunology, Autoimmunity:immunology, Demyelinating Autoimmune Diseases, CNS:immunology, Dendritic Cells:immunology, Humans, Mesenchymal Stromal Cells:immunology, T-Lymphocytes, Regulatory:immunology,.   

: Much experimental and clinical evidence has been accumulated indicating the complexity of regulatory processes associated with autoimmune demyelination. Even slight disbalance of immunoregulatory circuits may result in the loss of proper control of self antigen specific immune reaction. Here, we discuss the immunoregulatory potential of several immune (dendritic cells and regulatory T cells), as well as non-immune cell populations (mesenchymal stem cells and astrocytes) with regard to their possible role in autoimmune demyelination....

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Journal Article 2009; 30(2): 204-208 PubMed PMID: 19675523 Citation  Keywords:  Analysis of Variance, Animals, Bone Marrow Cells:physiology, Cell Movement:physiology, Cell Survival:physiology, Coculture Techniques:methods, Insulin:metabolism, Islets of Langerhans:physiology, Mesenchymal Stromal Cells:physiology, Microfluidic Analytic.   

OBJECTIVES: A coculture microfluidic chip had been fabricated to investigate the dynamic process of bone marrow mesenchymal stem cells migrating to the pancreatic islets in vitro.

METHODS: We fabricated a coculture microfluidic chip using standard procedures. On the chip, bone marrow mesenchymal stem cells and pancreatic islets were introduced respectively into two microchambers which could be connected by a traffic tunnel. Migration assay was performed along the tunnel, and the function of pancreatic islets was timely evaluated by analysis of insulin secretion in response to high-glucose-challenge.

RESULTS: The results showed that some bioactive factors excreted by freshly isolated pancreatic islets could enhance the migrating rate of BM-MSCs. Besides, pancreatic islets also showed a better survival and function by coculturing with bone marrow mesenchymal stem cells.

CONCLUSION: The results demonstrated that bone marrow mesenchymal stem cells had the ability to migrate to pancreatic islets and provide an apparent overall preservation for islet function. This microfluidic device was a potentially useful tool to mimic actual biological processes of bone marrow mesenchymal stem cells migrating to the pancreatic islets in vitro....

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Journal Article 2005; 26(5): 567-574 PubMed PMID: 16264399 Citation  Keywords:  Adenocarcinoma:metabolism, Adult, Aged, Breast Neoplasms:metabolism, Carcinoma, Squamous Cell:metabolism, Female, Head and Neck Neoplasms:metabolism, Humans, Immunohistochemistry, Lymphatic Metastasis, Male, Metallothionein:metabolism, Middle Aged, Stroma.   

INTRODUCTION: An accumulation of genetic alterations forming the field of cancerization is an important event for the transformation from normal to cancer cell in multistep carcinogenesis. Histopathologically healthy tumor adjacent tissue might be considered as a cancerization field which is typified by genetic changes required for the development of cancer. Metallothionein (MT) is considered to be a protective and anti-apoptotic protein. The aim of our study was to evaluate the MT expression in head and neck squamous cells carcinoma and breast adenocarcinoma and their histologically healthy adjacent tissue.

MATERIALS AND METHODS: We have sampled 29 tissue samples in total derived from head and neck cancers and 29 samples of their clear surgical margins, 33 breast adenocarcinomas and 33 clear surgical margins. Antibody recognizing MT-1 was used for immunohistochemical analysis.

RESULTS: MT expression was revealed in 85,7% of head and neck cancers and 94% of breast adenocarcinomas. It was found in all tumor adjacent tissue. MT expression was statistically significantly higher in tumor adjacent tissue than in cancer tissue in cases with the presence of lymph node metastases in both, breast adenocarcinoma and head and neck squamous cell carcinoma. Generally stroma seems to respond to the presence of cancer by the expression of MT, even in tissues which normally do not express MT.

CONCLUSIONS: MT might be a normal or protective reaction of healthy adjacent tissue to the presence of tumor....

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Case Reports 2018; 39(3): 149-155 PubMed PMID: 30431740 Citation  Keywords:  Adenocarcinoma:diagnostic imaging, Carcinoma, Non-Small-Cell Lung:diagnostic imaging, Duodenal Neoplasms:diagnostic imaging, Gastrointestinal Stromal Tumors:diagnostic imaging, Humans, Lung Neoplasms:diagnostic imaging, Male, Middle Aged, Neoplasms, Multi.   

: Neurofibromatosis type 1 (NF1, von Recklinghausen disease) is inherited in autosomal dominant way genetic disorder, with an incidence at birth 1:3000. It is one of the most common congenital disorders. It is characterized by café-au-lait spots, neurofibromas, and less common MPTST and gliomas of the optic nerve. It is caused by germline mutations of the NF1 gene, which acts as tumor suppressor. Inactivation of the gene leads to increased activation of the kinase pathways, and in consequence, uncontrolled proliferation of cells. The disease predisposes to the development of both benign and malignant tumors. Malignant tumors, but not related to the nervous system occur in neurofibromatosis quite rare. The aim of the study is a literature review of NF1, with presentation of a patient with NF1 and coexisting numerous tumors: synchronous somatostatinoma and gastrointestinal stromal tumor with metachronous prostate adenocarcinoma and non-small cell lung carcinoma. And attempt to answer the question if there is a common pathway for oncogenesis of these four tumors....

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