Neuronal expression of inducible nitric oxide synthase in hypothyroid rat.

Xu J, Xu Q, Chen X, Zou C, Zhao Z.
  Vol. 31 (6) 2010 Neuro endocrinology letters Journal Article   2010; 31(6): 848-851 PubMed PMID:  21196918    Citation  Keywords:  Age Factors, Animals, Animals, Newborn, Blotting, Western, Cerebral Cortex:metabolism, Disease Models, Animal, Female, Hypothyroidism:metabolism, Male, Mice, Mice, Inbred C57BL, Neurons:metabolism, Nitric Oxide Synthase Type II:genetics, Pregnancy, RNA, M.   

OBJECTIVES: The expression of iNOS mRNA and protein levels was studied in hypothyroid rat cerebral cortex during early days after postnatal life.

METHOD: The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis.

RESULT: ΔCt in hypothyroid group on day 7, 14, 21 was 18.89±0.92, 18.83±0.99, 16.48±0.29, separately. While in control group, ΔCt was 20.32±0.92, 20.07±0.86, 17.96±0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59 ± 5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37 ± 3%), compared with control group (58 ± 4%).

CONCLUSION: Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroid rat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.

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