Stimulatory effect of GH3 cell line conditioned medium on the proliferation of the endothelial cell line (HECa10) in vitro.

OBJECTIVES: The process of neoangiogenesis plays a key role in development and progression of solid tumours. The formation of new blood vessels different from the portal network system is important for pituitary tumour formation. The pivotal role of activated endothelial cells in angiogenesis was well documented. The degree of endothelial cells stimulation depends on the angiogenic activity of the tumorous cells. The aim of the study was to elucidate the angiogenic properties of the lactosomatotrophic pituitary cell line.

MATERIAL AND METHODS: The study was carried out on the mouse endothelial HECa10 cell line subjected to medium obtained from the culture of rat lactosomatotrophic pituitary GH3 cell line. The endothelial cell growth was measured using the modified colorimetric method EZ4Y detecting the viable cells. The secretion of vascular endothelial growth factor (VEGF) to GH3 cells conditioned medium was assessed by immunoenzymatic method.

RESULTS: It was shown that the exposure of endothelial cells to pituitary tumorous conditioned medium significantly stimulated the cellular growth after 24 and 48 hours of incubation. Moreover, the concentration of VEGF in GH3 conditioned medium occurred to be 13 times higher than in the control medium.

CONCLUSION: Our results indicate the angiogenic activity of lactosomatotrophic GH3 cell line and suggest the role of VEGF in pituitary tumorous angiogenesis.